منابع مشابه
Crystalline beef kidney rhodanese.
Although rhodanese activity occurs in many tissues (l-3), the enzyme had been obtained in crystalline form from beef liver only (4). Beef kidney rhodanese could not be crystallized by the same procedure (5). To extend the biochemical genetic comparison reported previously (5), however, it was desired to obtain the kidney enzyme in a very high state of purity. This paper reports the purification...
متن کاملMechanism of Rhodanese Catalysis of Thiosulfate-lipoate Oxidation-reduction.
Crystalline beef liver rhodanese (thiosulfate : cyanidesulfurtransferase, EC 2.8.1.1) catalyzes sulfur transfer from SSOato CNby a double displacement mechanism (3, 4). The biological role of this reaction is not clear, but its possible involvement in CNdetoxication has been repeatedly stressed. The present study of the specificity of the crystalline beef liver enzyme and of the occurrence of t...
متن کاملReversible Folding of Rhodanese
For the first time completely reversible unfolding was achieved for guanidinium chloride-denatured rhodanese using a systematically defined protocol. These conditions included fl-mercaptoethanol, lauryl maltoside, and sodium thiosulfate. All components were required to get more than the previous best reactivation with lauryl maltoside of 17% (Tandon, S., and Horowitz, P. (1986) J. Biol. Chem. 2...
متن کاملBeef Kidney 3-Hydroxyanthranilic Acid Oxygenase
Beef kidney 3-hydroxyanthranilic acid oxygenase has been purified to homogeneity. It is a single subunit protein of M, = 34,000 * 2,000 with a frictional coefficient (f/iO) of about 1.1. The enzyme readily aggregates to form, apparently inactive, higher molecular weight oligomers. The very rapid loss of enzyme activity during the assay was analyzed extensively. It was found to be due to inactiv...
متن کاملThe rhodanese/Cdc25 phosphatase superfamily
Rhodanese domains are ubiquitous structural modules occurring in the three major evolutionary phyla. They are found as tandem repeats, with the C-terminal domain hosting the properly structured active-site Cys residue, as single domain proteins or in combination with distinct protein domains. An increasing number of reports indicate that rhodanese modules are versatile sulfur carriers that have...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69808-4